|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CHN1 ORF mammalian expression plasmid, C-GFPSpark tag||HG14914-ACG|
|Human CHN1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14914-ACR|
|Human CHN1 ORF mammalian expression plasmid, N-GFPSpark tag||HG14914-ANG|
|Human CHN1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14914-ANR|
|Human CHN1 ORF mammalian expression plasmid, C-Flag tag||HG14914-CF|
|Human CHN1 ORF mammalian expression plasmid, C-His tag||HG14914-CH|
|Human CHN1 ORF mammalian expression plasmid, C-Myc tag||HG14914-CM|
|Human CHN1 ORF mammalian expression plasmid, C-HA tag||HG14914-CY|
|Human CHN1 Gene cDNA clone plasmid||HG14914-G|
|Human CHN1 ORF mammalian expression plasmid, N-Flag tag||HG14914-NF|
|Human CHN1 ORF mammalian expression plasmid, N-His tag||HG14914-NH|
|Human CHN1 ORF mammalian expression plasmid, N-Myc tag||HG14914-NM|
|Human CHN1 ORF mammalian expression plasmid, N-HA tag||HG14914-NY|
|Human CHN1 natural ORF mammalian expression plasmid||HG14914-UT|
|Learn more about expression Vectors|
CHN1, also known as chimerin 1, is a TPase-activating protein for ras-related p21-rac and a phorbol ester receptor. It is predominantly expressed in neurons, and plays an important role in neuronal signal-transduction mechanisms. CHN1 is involved in the assembly of neuronal locomotor circuits as a direct effector of EPHA4 in axon guidance. The CHN1 gene provides instructions for making two very similar proteins called α1-chimaerin and α2-chimaerin. These proteins play an important role in the early development of the nervous system. In particular, they help regulate complex chemical signaling pathways during the formation and development of nerve cells (neurons). These proteins help guide the growth of axons and dendrites, which are specialized extensions of neurons that transmit and receive nerve impulses throughout the nervous system.