|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SCLY ORF mammalian expression plasmid, C-GFPSpark tag||HG14905-ACG|
|Human SCLY ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14905-ACR|
|Human SCLY ORF mammalian expression plasmid, N-GFPSpark tag||HG14905-ANG|
|Human SCLY ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14905-ANR|
|Human SCLY ORF mammalian expression plasmid, C-Flag tag||HG14905-CF|
|Human SCLY ORF mammalian expression plasmid, C-His tag||HG14905-CH|
|Human SCLY ORF mammalian expression plasmid, C-Myc tag||HG14905-CM|
|Human SCLY ORF mammalian expression plasmid, C-HA tag||HG14905-CY|
|Human SCLY Gene cDNA clone plasmid||HG14905-G|
|Human SCLY ORF mammalian expression plasmid, N-Flag tag||HG14905-NF|
|Human SCLY ORF mammalian expression plasmid, N-His tag||HG14905-NH|
|Human SCLY ORF mammalian expression plasmid, N-Myc tag||HG14905-NM|
|Human SCLY ORF mammalian expression plasmid, N-HA tag||HG14905-NY|
|Human SCLY natural ORF mammalian expression plasmid||HG14905-UT|
|Learn more about expression Vectors|
SCLY, also known as selenocysteine lyase, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. It is a novel enzyme that exclusively decomposes L-selenocysteine into L-alanine and H2Se in various mammalian tissues. SCLY contains pyridoxal 5'-phosphate and weighs approximately 85,000. SCLY participates in selenoamino acid metabolism. It employs one cofactor, pyridoxal phosphate. Its maximum reactivity is at about pH 9.0. It was shown that 1 mol of selenocysteine is converted to equimolar amounts of alanine and H2Se. The following amino acids are insert: L-cysteine, L-serine, L-cysteine sulfinate, selenocysteamine, Se-ethyl-DL-selenocysteine, and L-selenohomocysteine. L-Cysteine (Ki, 1.0 mM) competes with L-selenocysteine (Km, 0.83mM) to inhibit the enzyme reaction.