|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human S100B ORF mammalian expression plasmid, C-GFPSpark tag||HG10181-ACG|
|Human S100B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10181-ACR|
|Human S100B ORF mammalian expression plasmid, N-GFPSpark tag||HG10181-ANG|
|Human S100B ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10181-ANR|
|Human S100B ORF mammalian expression plasmid, C-Flag tag||HG10181-CF|
|Human S100B ORF mammalian expression plasmid, C-His tag||HG10181-CH|
|Human S100B ORF mammalian expression plasmid, C-Myc tag||HG10181-CM|
|Human S100B ORF mammalian expression plasmid, C-HA tag||HG10181-CY|
|Human S100B Gene cDNA clone plasmid||HG10181-M|
|Human S100B ORF mammalian expression plasmid, Flag tag||HG10181-M-F|
|Human S100B ORF mammalian expression plasmid, N-Flag tag||HG10181-NF|
|Human S100B ORF mammalian expression plasmid, N-His tag||HG10181-NH|
|Human S100B ORF mammalian expression plasmid, N-Myc tag||HG10181-NM|
|Human S100B ORF mammalian expression plasmid, N-HA tag||HG10181-NY|
|Human S100B natural ORF mammalian expression plasmid||HG10181-UT|
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S100B is a member of the S100 family of proteins containing two EF-hand-type calcium-binding motifs. S100B exerts both intracellular and extracellular functions. Intracellular S100B acts as a stimulator of cell proliferation and migration and an inhibitor of apoptosis and differentiation, which might have important implications during brain, cartilage and skeletal muscle development and repair, activation of astrocytes in the course of brain damage and neurodegenerative processes, and of cardiomyocyte remodeling after infarction, as well as in melanomagenesis and gliomagenesis. As an extracellular factor, S100B engages RAGE (receptor for advanced glycation end products) in a variety of cell types with different outcomes (i.e. beneficial or detrimental, pro-proliferative or pro-differentiative) depending on the concentration attained by the protein, the cell type and the microenvironment. This calcium binding astrocyte-specific cytokine, presents a marker of astrocytic activation and reflects CNS injury. The excellent sensitivity of S100B has enabled it to confirm the existence of subtle brain injury in patients with mild head trauma, strokes, and after successful resuscitation from cardiopulmonary arrest. Recent findings provide evidence, that S100B may decrease neuronal injury and/or contribute to repair following traumatic brain injury (TBI). Hence, S100B, far from being a negative determinant of outcome, as suggested previously in the human TBI and ischemia literature, is of potential therapeutic value that could improve outcome in patients who sustain various forms of acute brain damage.