|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human CD221 / IGF1R ORF mammalian expression plasmid, C-GFPSpark tag||HG10164-ACG|
|Human CD221 / IGF1R ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10164-ACR|
|Human CD221 / IGF1R ORF mammalian expression plasmid, C-Flag tag||HG10164-CF|
|Human CD221 / IGF1R ORF mammalian expression plasmid, C-His tag||HG10164-CH|
|Human CD221 / IGF1R ORF mammalian expression plasmid, C-Myc tag||HG10164-CM|
|Human CD221 / IGF1R ORF mammalian expression plasmid, C-HA tag||HG10164-CY|
|Human CD221 / IGF1R Gene cDNA clone plasmid||HG10164-M|
|Human CD221 / IGF1R ORF mammalian expression plasmid, N-Flag tag||HG10164-NF|
|Human CD221 / IGF1R ORF mammalian expression plasmid, N-His tag||HG10164-NH|
|Human CD221 / IGF1R ORF mammalian expression plasmid, N-Myc tag||HG10164-NM|
|Human CD221 / IGF1R ORF mammalian expression plasmid, N-HA tag||HG10164-NY|
|Human CD221 / IGF1R natural ORF mammalian expression plasmid||HG10164-UT|
|Learn more about expression Vectors|
The insulin-like growth factor-1 receptor (IGF1R) is a transmembrane tyrosine kinase involved in several biological processes including cell proliferation, differentiation, DNA repair, and cell survival. This a disulfide-linked heterotetrameric transmembrane protein consisting of two α and two β subunits, and among which, the α subunit is extracellular while the β subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF1R signalling pathway is activated in the mammalian nervous system from early developmental stages. Its major effect on developing neural cells is to promote their growth and survival. This pathway can integrate its action with signalling pathways of growth and morphogenetic factors that induce cell fate specification and selective expansion of specified neural cell subsets. Modulation of cell migration is another possible role that IGF1R activation may play in neurogenesis. In the mature brain, IGF-I binding sites have been found in different regions of the brain, and multiple reports confirmed a strong neuroprotective action of the IGF-IR against different pro-apoptotic insults. IGF1R is an important signaling molecule in cancer cells and plays an essential role in the establishment and maintenance of the transformed phenotype. Inhibition of IGF1R signaling thus appears to be a promising strategy to interfere with the growth and survival of cancer cells. IGF1R is frequently overexpressed by tumours, and mediates proliferation and apoptosis protection. IGF signalling also influences hypoxia signalling, protease secretion, tumour cell motility and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, the IGF1R is now an attractive anti-cancer treatment target.