|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Human MICA ORF mammalian expression plasmid, C-GFPSpark tag||HG12302-ACG|
|Human MICA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12302-ACR|
|Human MICA ORF mammalian expression plasmid, C-Flag tag||HG12302-CF|
|Human MICA ORF mammalian expression plasmid, C-His tag||HG12302-CH|
|Human MICA ORF mammalian expression plasmid, C-Myc tag||HG12302-CM|
|Human MICA ORF mammalian expression plasmid, C-HA tag||HG12302-CY|
|Human MICA Gene cDNA clone plasmid||HG12302-G|
|Human MICA ORF mammalian expression plasmid, N-Flag tag||HG12302-NF|
|Human MICA ORF mammalian expression plasmid, N-His tag||HG12302-NH|
|Human MICA ORF mammalian expression plasmid, N-Myc tag||HG12302-NM|
|Human MICA ORF mammalian expression plasmid, N-HA tag||HG12302-NY|
|Human MICA natural ORF mammalian expression plasmid||HG12302-UT|
|Learn more about expression Vectors|
MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gammadelta T cells, CD8+ alphabeta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gammadelta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems.