|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human IL18 ORF mammalian expression plasmid, C-GFPSpark tag||HG10119-ACG|
|Human IL18 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10119-ACR|
|Human IL18 ORF mammalian expression plasmid, C-Flag tag||HG10119-CF|
|Human IL18 ORF mammalian expression plasmid, C-His tag||HG10119-CH|
|Human IL18 ORF mammalian expression plasmid, C-Myc tag||HG10119-CM|
|Human IL18 ORF mammalian expression plasmid, C-HA tag||HG10119-CY|
|Human IL18 Gene cDNA clone plasmid||HG10119-M|
|Human IL18 ORF mammalian expression plasmid, N-Flag tag||HG10119-NF|
|Human IL18 ORF mammalian expression plasmid, N-His tag||HG10119-NH|
|Human IL18 ORF mammalian expression plasmid, N-Myc tag||HG10119-NM|
|Human IL18 ORF mammalian expression plasmid, N-HA tag||HG10119-NY|
|Human IL18 natural ORF mammalian expression plasmid||HG10119-UT|
|Learn more about expression Vectors|
Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.