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PPM1A / PP2C-alpha Antibody (FITC), Mouse MAb

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PPM1AAntibody Product Information
Immunogen:Recombinant Human PPM1A / PP2C-alpha protein (Catalog#12984-H08E)
Clone ID:01
Ig Type:Mouse IgG2a
Concentration:10 μl/Test, 0.1 mg/ml
Formulation:Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PPM1A / PP2C-alpha (rh PPM1A / PP2C-alpha; Catalog#12984-H08E; P35813-1; Met1-Trp382) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
PPM1AAntibody Usage Guide
Specificity:Human PPM1A / PP2C-alpha
Application:FCM
Storage:This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Images
PPM1A / PP2C-alpha Antibody (FITC), Mouse MAb, Flow cytometric
[Click to enlarge image]
Caption:
Flow cytometric analysis of Human PPM1A expression in Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Mouse anti-Human PPM1A. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Background

Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.

References
  • Lin X, et al. (2006) PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell. 125(5): 915-28.
  • Marc F, et al. (2003) Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3. Proc Natl Acad. 100 (26): 16006-11.
  • Mann DJ, et al. (1992) Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim Biophys Acta. 1130 (1): 100-4.
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    Catalog:12984-MM01-F-50
    List Price: $130.00  (Save $0.00)
    Price:$130.00      [How to order]
    AvailabilityIn Stock
    Images
    • PPM1A / PP2C-alpha Antibody (FITC), Mouse MAb, Flow cytometric
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