|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Human A2M ORF mammalian expression plasmid, C-GFPSpark tag||HG10952-ACG|
|Human A2M ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10952-ACR|
|Human A2M ORF mammalian expression plasmid, C-Flag tag||HG10952-CF|
|Human A2M ORF mammalian expression plasmid, C-His tag||HG10952-CH|
|Human A2M ORF mammalian expression plasmid, C-Myc tag||HG10952-CM|
|Human A2M ORF mammalian expression plasmid, C-HA tag||HG10952-CY|
|Human A2M Gene cDNA clone plasmid||HG10952-M|
|Human A2M natural ORF mammalian expression plasmid||HG10952-M-N|
|Human A2M ORF mammalian expression plasmid, N-Flag tag||HG10952-NF|
|Human A2M ORF mammalian expression plasmid, N-His tag||HG10952-NH|
|Human A2M ORF mammalian expression plasmid, N-Myc tag||HG10952-NM|
|Human A2M ORF mammalian expression plasmid, N-HA tag||HG10952-NY|
|Human A2M natural ORF mammalian expression plasmid||HG10952-UT|
|Learn more about expression Vectors|
alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is an abundant protein of the plasma of vertebrates and members of several invertebrate phyla and functions as a broad-spectrum protease-binding protein. alpha-2-macroglobulin is produced by the liver, and is a major component of the alpha-2 band in protein electrophoresis. alpha-2-macroglobulin is a large plasma glycoprotein that has long been known as an irreversible inhibitor of a variety of proteinases. More recently, it has been reported that numerous growth factors, cytokines and hormones bind to alpha 2M through diverse mechanisms. A2M is also produced in the brain where it binds multiple extracellular ligands and is internalized by neurons and astrocytes. In the brain of Alzheimer's disease (AD) patients, A2M has been localized to diffuse amyloid plaques. A2M also binds soluble beta-amyloid, of which it mediates degradation. Protease-conjugated alpha2-macroglobulin is selectively bound by cells contacting the body fluids and alpha2-macroglobulin and its protease cargo are then internalized and degraded in secondary lysosomes of those cells. In addition to this function as an agent for protease clearance, alpha2-macroglobulin binds a variety of other ligands, including several peptide growth factors and modulates the activity of a lectin-dependent cytolytic pathway in arthropods.