|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human EPHB3 ORF mammalian expression plasmid, C-GFPSpark tag||HG13925-ACG|
|Human EPHB3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13925-ACR|
|Human EPHB3 ORF mammalian expression plasmid, C-Flag tag||HG13925-CF|
|Human EPHB3 ORF mammalian expression plasmid, C-His tag||HG13925-CH|
|Human EPHB3 ORF mammalian expression plasmid, C-Myc tag||HG13925-CM|
|Human EPHB3 ORF mammalian expression plasmid, C-HA tag||HG13925-CY|
|Human EPHB3 Gene cDNA clone plasmid||HG13925-G|
|Human EPHB3 ORF mammalian expression plasmid, N-Flag tag||HG13925-NF|
|Human EPHB3 ORF mammalian expression plasmid, N-His tag||HG13925-NH|
|Human EPHB3 ORF mammalian expression plasmid, N-Myc tag||HG13925-NM|
|Human EPHB3 ORF mammalian expression plasmid, N-HA tag||HG13925-NY|
|Human EPHB3 natural ORF mammalian expression plasmid||HG13925-UT|
|Learn more about expression Vectors|
Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.