|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human MVK ORF mammalian expression plasmid, C-GFPSpark tag||HG13923-ACG|
|Human MVK ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13923-ACR|
|Human MVK ORF mammalian expression plasmid, N-GFPSpark tag||HG13923-ANG|
|Human MVK ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13923-ANR|
|Human MVK ORF mammalian expression plasmid, C-Flag tag||HG13923-CF|
|Human MVK ORF mammalian expression plasmid, C-His tag||HG13923-CH|
|Human MVK ORF mammalian expression plasmid, C-Myc tag||HG13923-CM|
|Human MVK ORF mammalian expression plasmid, C-HA tag||HG13923-CY|
|Human MVK Gene cDNA clone plasmid||HG13923-G|
|Human MVK ORF mammalian expression plasmid, N-Flag tag||HG13923-NF|
|Human MVK ORF mammalian expression plasmid, N-His tag||HG13923-NH|
|Human MVK ORF mammalian expression plasmid, N-Myc tag||HG13923-NM|
|Human MVK ORF mammalian expression plasmid, N-HA tag||HG13923-NY|
|Human MVK natural ORF mammalian expression plasmid||HG13923-UT|
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Mevalonate kinase belongs to the GHMP kinase family, Mevalonate kinase subfamily. It can be found in a wide variety of organisms from bacteria to mammals. Mevalonate kinase may be a regulatory site in cholesterol biosynthetic pathway. Defects in mevalonate kinase can cause mevalonic aciduria (MEVA). It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy, and ataxia. Defects in mevalonate kinase can also cause hyperimmunoglobulinemia D and periodic fever syndrome (HIDS). HIDS is an autosomal recessive disease characterized by recurrent episodes of unexplained high fever associated with skin rash, diarrhea, adenopathy (swollen, tender lymph nodes), athralgias and/or arthritis.