|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ETHE1 ORF mammalian expression plasmid, C-GFPSpark tag||HG14681-ACG|
|Human ETHE1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14681-ACR|
|Human ETHE1 ORF mammalian expression plasmid, N-GFPSpark tag||HG14681-ANG|
|Human ETHE1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14681-ANR|
|Human ETHE1 ORF mammalian expression plasmid, C-Flag tag||HG14681-CF|
|Human ETHE1 ORF mammalian expression plasmid, C-His tag||HG14681-CH|
|Human ETHE1 ORF mammalian expression plasmid, C-Myc tag||HG14681-CM|
|Human ETHE1 ORF mammalian expression plasmid, C-HA tag||HG14681-CY|
|Human ETHE1 Gene cDNA clone plasmid||HG14681-G|
|Human ETHE1 ORF mammalian expression plasmid, N-Flag tag||HG14681-NF|
|Human ETHE1 ORF mammalian expression plasmid, N-His tag||HG14681-NH|
|Human ETHE1 ORF mammalian expression plasmid, N-Myc tag||HG14681-NM|
|Human ETHE1 ORF mammalian expression plasmid, N-HA tag||HG14681-NY|
|Human ETHE1 natural ORF mammalian expression plasmid||HG14681-UT|
|Learn more about expression Vectors|
ETHE1, also known as HSCO, is a sulfur dioxygenase that localizes within the mitochondrial matrix. ETHE1 probably plays an important role in metabolic homeostasis in mitochondria. It may also function as a nuclear-cytoplasmic shuttling protein that binds transcription factor RELA/NFKB3 in the nucleus and exports it to the cytoplasm. ETHE1 can suppresses p53-induced apoptosis by preventing nuclear localization of RELA. Mutations in ETHE1 gene result in ethylmalonic encephalopathy. Ethylmalonic encephalopathy is an autosomal recessive, invariably fatal disorder characterized by early-onset encephalopathy, microangiopathy, chronic diarrhea, defective cytochrome c oxidase (COX) in muscle and brain, high concentrations of C4 and C5 acylcarnitines in blood and high excretion of ethylmalonic acid in urine.