|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CABP5 ORF mammalian expression plasmid, C-GFPSpark tag||HG13896-ACG|
|Human CABP5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13896-ACR|
|Human CABP5 ORF mammalian expression plasmid, N-GFPSpark tag||HG13896-ANG|
|Human CABP5 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13896-ANR|
|Human CABP5 ORF mammalian expression plasmid, C-Flag tag||HG13896-CF|
|Human CABP5 ORF mammalian expression plasmid, C-His tag||HG13896-CH|
|Human CABP5 ORF mammalian expression plasmid, C-Myc tag||HG13896-CM|
|Human CABP5 ORF mammalian expression plasmid, C-HA tag||HG13896-CY|
|Human CABP5 Gene cDNA clone plasmid||HG13896-G|
|Human CABP5 ORF mammalian expression plasmid, N-Flag tag||HG13896-NF|
|Human CABP5 ORF mammalian expression plasmid, N-His tag||HG13896-NH|
|Human CABP5 ORF mammalian expression plasmid, N-Myc tag||HG13896-NM|
|Human CABP5 ORF mammalian expression plasmid, N-HA tag||HG13896-NY|
|Human CABP5 natural ORF mammalian expression plasmid||HG13896-UT|
|Learn more about expression Vectors|
CABP3, also known as CABP5, belongs to a subfamily of calcium binding proteins, which share similarity to calmodulin. Calcium binding proteins are an important component of calcium mediated cellular signal transduction. Expression of CABP3 gene is retina-specific. The mouse homolog of CABP3 has been shown to express in the inner nuclear layer of the retina, suggested its role in neuronal functioning. The specific function of CABP3 gene is unknown. Study of the transcripts and genomic structure revealed that the 5 end of this gene is complementary and reverse to that of the CABP5 gene, and the sequence beyond the overlapping region is nearly identical to that of CABP5. Thus, these two genes encode the protein products with distinct N-terminal halves but identical C-terminal halves. CABP3 inhibits calcium-dependent inactivation of L-type calcium channel and shifts voltage dependence of activation to more depolarized membrane potentials. It is also involved in the transmission of light signals.