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Human CDC2 & CCNE1 Heterotrimer Protein

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Human CDC2 & CCNE1 Protein Product Information
Synonym:CDC2, CDK1, CDC28A, CDKN1, P34CDC2 & CCNE1, CCNE
Protein Construction:A DNA sequence encoding the human CDC2 (NP_001777.1) (Met1-Met297) was expressed with a GST tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNE1 (NP_001229.1) (Met1-Ala410) was expressed with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterotrimer was purified.
Expressed Host:Baculovirus-Insect cells
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human CDC2 & CCNE1 Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Bio-Activity:The specific activity was determined to be 150 nmol/min/mg using Histone H1 as substrate.
Endotoxin:<1.0 EU per μg protein as determined by the LAL method.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met & His
Molecule Mass:The recombinant heterotrimer of human CDC2 & CCNE1 comprises 950 (522+428) amino acids and has a calculated molecular mass of 109.7 (60.4+49.3) kDa.
Formulation:Supplied as sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 8.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CDC2 & CCNE1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human CDC2 & CCNE1 Protein SDS-PAGE
Human CDC2 & CCNE1 Heterotrimer Protein SDS-PAGE
Other CDC2 & CCNE1 Recombinant Protein Products
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Catalog: CT027-H0907B-20
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All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.