|Datasheet||Specific References||Reviews||Related Products||Protocols|
|EGF R, ErbB, HER1, mENA, ErbB1, PIG61|
|A DNA sequence encoding the cytoplasmic domain (Met 668-Ala 1210) of human EGFR (NP_005219) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.|
|Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.|
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
|> 85 % as determined by SDS-PAGE|
|The specific activity was determined to be 105 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate (see Activity Assay Protocol)|
|<1.0 EU per μg protein as determined by the LAL method.|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The recombinant human EGFR /GST chimera consists of 780 amino acids and has a calculated molecular mass of 89.1 kDa.|
|Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.