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Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-tagged

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SMAD5cDNA Clone Product Information
Gene_bank_ref_id:NM_001001420.1
cDNA Size:1398
cDNA Description:ORF Clone of Homo sapiens SMAD family member 5 (SMAD5), transcript variant 3 DNA.
Gene Synonym:Smad5, Dwfc, JV5-1, MADH5, DKFZp781C1895
Species:Human
Vector:pCMV3-C-HA
Restriction Site:
Tag Sequence:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-C-HA Vector Information
 
Vector Name pCMV3-C-HA
Vector Size 6161bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag HA
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-C-HA Physical Map
Schematic of pCMV3-C-HA Multiple Cloning Sites

HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-tagged on other vectors
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10017-ACG$325
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10017-ACR$325
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10017-ANG$325
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10017-ANR$325
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10017-CF$295
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10017-CH$295
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10017-CM$295
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10017-CY$295
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone)HG10017-M$95
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-taggedHG10017-M-F$295
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10017-M-N$295
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10017-NF$295
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10017-NH$295
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10017-NM$295
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10017-NY$295
Human SMAD5 transcript variant 3 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10017-UT$295
 Learn more about expression Vectors
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Background

SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

References
  • Vinayagam A. et al., 2011, Sci Signal. 4 (189): rs8.
  • Sangadala S. et al., 2007, J Biomol Struct Dyn. 25 (1): 11-23.
  • Riggins GJ. et al., 1996, Nat Genet. 13 (3): 347-9.
  • Size / Price
    Catalog:HG10017-CY
    List Price: $295.00  (Save $0.00)
    Price:$295.00      [How to order]
    Availability2-3 weeks
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