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Cynomolgus Autotaxin / ENPP2 Protein (His Tag)

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Cynomolgus ENPP2 Protein Product Information
Synonym:Autotaxin, ENPP2
Protein Construction:A DNA sequence encoding the cynomolgus ENPP2 (XP_005564040.1) (Asp49-Ile863) was expressed with a polyhistidine tag at the N-terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Cynomolgus ENPP2 Protein QC Testing
Purity:> 85 % as determined by SDS-PAGE.
Bio-Activity:Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP).
The specific activity is > 1,000 pmol/min/µg.
Endotoxin:<1.0 EU per μg protein as determined by the LAL method.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:His
Molecule Mass:The recombinant cynomolgus ENPP2 consists 834 amino acids and predicts a molecular mass of 96 kDa.
Formulation:Lyophilized from sterile PBS,pH7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Cynomolgus ENPP2 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Cynomolgus ENPP2 Protein SDS-PAGE
Cynomolgus Autotaxin / ENPP2 Protein (His Tag) SDS-PAGE
Cynomolgus Autotaxin / ENPP2 Protein (His Tag) SDS-PAGE
Cynomolgus Autotaxin / ENPP2 Protein (His Tag) SDS-PAGE
Other ENPP2 Recombinant Protein Products
Autotaxin/ENPP2 Background

ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2), also referred as Autotaxin, is a secreted enzyme encoded by the ENPP2 gene. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. ATX has been found to catalyze the formation of cyclic phosphatidic acid (cPA), which have antitumor role by antimitogenic regulation of cell cycle, inhibition of cancer invasion and metastasis. LPA receptors and ATX are upregulated in numerous cancer cell types and show expression patterns that correlate with tumor cell invasiveness. Thus, Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection.

Cynomolgus Autotaxin/ENPP2 References
  • Tania M, et al. (2010) Autotaxin: a protein with two faces. Biochem Biophys Res Commun. 401(4): 493-7.
  • Ikeda H, et al. (2009) Significance of serum autotaxin activity in gastrointestinal disease. Rinsho Byori. 57(5): 445-9.
  • Parrill AL, et al. (2008) Autotaxin inhibition: challenges and progress toward novel anti-cancer agents. Anticancer Agents Med Chem. 8(8): 917-23.
  • Pradere J.P, et al. (2007) Secretion and lysophospholipase D activity of autotaxin by adipocytes are controlled by N-glycosylation and signal peptidase. Biochim Biophys Acta. 1771: 93-102.
  • Boucher J, et al. (2005) Potential involvement of adipocyte insulin resistance in obesity-associated up-regulation of adipocyte lysophospholipase D/autotaxin expression. Diabetologia. 48: 569-77.
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    Catalog: 90223-C07H-10
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