|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Human PI3 ORF mammalian expression plasmid, C-GFPSpark tag||HG12187-ACG|
|Human PI3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12187-ACR|
|Human PI3 ORF mammalian expression plasmid, C-Flag tag||HG12187-CF|
|Human PI3 ORF mammalian expression plasmid, C-His tag||HG12187-CH|
|Human PI3 ORF mammalian expression plasmid, C-Myc tag||HG12187-CM|
|Human PI3 ORF mammalian expression plasmid, C-HA tag||HG12187-CY|
|Human PI3 Gene cDNA clone plasmid||HG12187-G|
|Human PI3 ORF mammalian expression plasmid, N-Flag tag||HG12187-NF|
|Human PI3 ORF mammalian expression plasmid, N-His tag||HG12187-NH|
|Human PI3 ORF mammalian expression plasmid, N-Myc tag||HG12187-NM|
|Human PI3 ORF mammalian expression plasmid, N-HA tag||HG12187-NY|
|Human PI3 natural ORF mammalian expression plasmid||HG12187-UT|
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Elafin, also known as Elastase-specific inhibitor, Peptidase inhibitor 3, Protease inhibitor WAP3, Skin-derived antileukoproteinase, WAP four-disulfide core domain protein 14, PI3, WAP3 and WFDC14, is a secreted protein which contains one WAP domain. Elafin / PI3 consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain at the N-terminus serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins. The serine anti-protease Elafin / PI3 is expressed by monocytes, alveolar macrophages, neutrophils, and at mucosal surfaces and possesses antimicrobial activity. It is also known to reduce lipopolysaccharide-induced neutrophil influx into murine alveoli as well as to abrogate lipopolysaccharide-induced production of matrix metalloprotease 9, macrophage inhibitory protein 2, and tumor necrosis factor-alpha by as-yet unidentified mechanisms. Elafin / PI3 is a neutrophil serine protease inhibitor expressed in lung and displaying anti-inflammatory and anti-bacterial properties. Elafin / PI3 is a neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Elafin / PI3 will regulate proteolytic enzymes during menstruation and will contribute to the innate defense against uterine infection.