|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human KCNIP3 ORF mammalian expression plasmid, C-GFPSpark tag||HG14683-ACG|
|Human KCNIP3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14683-ACR|
|Human KCNIP3 ORF mammalian expression plasmid, C-Flag tag||HG14683-CF|
|Human KCNIP3 ORF mammalian expression plasmid, C-His tag||HG14683-CH|
|Human KCNIP3 ORF mammalian expression plasmid, C-Myc tag||HG14683-CM|
|Human KCNIP3 ORF mammalian expression plasmid, C-HA tag||HG14683-CY|
|Human KCNIP3 Gene cDNA clone plasmid||HG14683-G|
|Human KCNIP3 ORF mammalian expression plasmid, N-Flag tag||HG14683-NF|
|Human KCNIP3 ORF mammalian expression plasmid, N-His tag||HG14683-NH|
|Human KCNIP3 ORF mammalian expression plasmid, N-Myc tag||HG14683-NM|
|Human KCNIP3 ORF mammalian expression plasmid, N-HA tag||HG14683-NY|
|Human KCNIP3 natural ORF mammalian expression plasmid||HG14683-UT|
|Learn more about expression Vectors|
CSEN, also known as calsenilin and KCNIP3, is a member of the family of voltage-gated potassium (Kv) channel-interacting proteins, which belong to the recoverin branch of the EF-hand superfamily. Members of this family are integral subunit components of native Kv4 channel complexes that may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. CSEN also functions as a calcium-regulated transcriptional repressor, and interacts with presenilins. CSEN binds to the DRE element of genes including PDYN and FOS.