|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Human GRN ORF mammalian expression plasmid, C-GFPSpark tag||HG10826-ACG|
|Human GRN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10826-ACR|
|Human GRN ORF mammalian expression plasmid, C-Flag tag||HG10826-CF|
|Human GRN ORF mammalian expression plasmid, C-His tag||HG10826-CH|
|Human GRN ORF mammalian expression plasmid, C-Myc tag||HG10826-CM|
|Human GRN ORF mammalian expression plasmid, C-HA tag||HG10826-CY|
|Human GRN Gene cDNA clone plasmid||HG10826-M|
|Human GRN ORF mammalian expression plasmid, Myc tag||HG10826-M-M|
|Human GRN ORF mammalian expression plasmid, N-Flag tag||HG10826-NF|
|Human GRN ORF mammalian expression plasmid, N-His tag||HG10826-NH|
|Human GRN ORF mammalian expression plasmid, N-Myc tag||HG10826-NM|
|Human GRN ORF mammalian expression plasmid, N-HA tag||HG10826-NY|
|Human GRN natural ORF mammalian expression plasmid||HG10826-UT|
|Learn more about expression Vectors|
&Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Granulin expression inhibited Tat transactivation, and tethering experiments showed that this effect was due, at least in part, to a direct action on cyclin T1 in the absence of Tat.