|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Canine IL3RA ORF mammalian expression plasmid, C-GFPSpark tag||DG70077-ACG|
|Canine IL3RA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70077-ACR|
|Canine IL3RA ORF mammalian expression plasmid, C-Flag tag||DG70077-CF|
|Canine IL3RA ORF mammalian expression plasmid, C-His tag||DG70077-CH|
|Canine IL3RA ORF mammalian expression plasmid, C-Myc tag||DG70077-CM|
|Canine IL3RA ORF mammalian expression plasmid, C-HA tag||DG70077-CY|
|Canine IL3RA Gene cDNA clone plasmid||DG70077-G|
|Canine IL3RA ORF mammalian expression plasmid, N-Flag tag||DG70077-NF|
|Canine IL3RA ORF mammalian expression plasmid, N-His tag||DG70077-NH|
|Canine IL3RA ORF mammalian expression plasmid, N-Myc tag||DG70077-NM|
|Canine IL3RA ORF mammalian expression plasmid, N-HA tag||DG70077-NY|
|Canine IL3RA natural ORF mammalian expression plasmid||DG70077-UT|
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Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and / or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.