|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human PDE1C ORF mammalian expression plasmid, C-GFPSpark tag||HG13605-ACG|
|Human PDE1C ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13605-ACR|
|Human PDE1C ORF mammalian expression plasmid, N-GFPSpark tag||HG13605-ANG|
|Human PDE1C ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13605-ANR|
|Human PDE1C ORF mammalian expression plasmid, C-Flag tag||HG13605-CF|
|Human PDE1C ORF mammalian expression plasmid, C-His tag||HG13605-CH|
|Human PDE1C ORF mammalian expression plasmid, C-Myc tag||HG13605-CM|
|Human PDE1C ORF mammalian expression plasmid, C-HA tag||HG13605-CY|
|Human PDE1C Gene cDNA clone plasmid||HG13605-G|
|Human PDE1C ORF mammalian expression plasmid, N-Flag tag||HG13605-NF|
|Human PDE1C ORF mammalian expression plasmid, N-His tag||HG13605-NH|
|Human PDE1C ORF mammalian expression plasmid, N-Myc tag||HG13605-NM|
|Human PDE1C ORF mammalian expression plasmid, N-HA tag||HG13605-NY|
|Human PDE1C natural ORF mammalian expression plasmid||HG13605-UT|
|Learn more about expression Vectors|
PDE1C belongs to the cyclic nucleotide phosphodiesterase family, PDE1 subfamily. Phosphodiesterases (PDEs) are a family of related phosphohydrolyases that selectively catalyze the hydrolysis of 3' cyclic phosphate bonds in adenosine and/or guanine 3',5' cyclic monophosphate (cAMP and/or cGMP). They regulate the cellular levels, localization and duration of action of these second messengers by controlling the rate of their degradation. PDEs are expressed ubiquitously, with each subtype having a specific tissue distribution. These enzymes are involved in many signal transduction pathways and their functions include vascular smooth muscle proliferation and contraction, cardiac contractility, platelet aggregation, hormone secretion, immune cell activation, and they are involved in learning and memory. PDE1C has a high affinity for both cAMP and cGMP. It is expressed in several tissues, including brain and heart. As a cyclic nucleotide phosphodiesterase, PDE1C has a dual-specificity for the second messengers cAMP and cGMP.