|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CKB ORF mammalian expression plasmid, C-GFPSpark tag||HG14415-ACG|
|Human CKB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14415-ACR|
|Human CKB ORF mammalian expression plasmid, N-GFPSpark tag||HG14415-ANG|
|Human CKB ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14415-ANR|
|Human CKB ORF mammalian expression plasmid, C-Flag tag||HG14415-CF|
|Human CKB ORF mammalian expression plasmid, C-His tag||HG14415-CH|
|Human CKB ORF mammalian expression plasmid, C-Myc tag||HG14415-CM|
|Human CKB ORF mammalian expression plasmid, C-HA tag||HG14415-CY|
|Human CKB Gene cDNA clone plasmid||HG14415-G|
|Human CKB ORF mammalian expression plasmid, N-Flag tag||HG14415-NF|
|Human CKB ORF mammalian expression plasmid, N-His tag||HG14415-NH|
|Human CKB ORF mammalian expression plasmid, N-Myc tag||HG14415-NM|
|Human CKB ORF mammalian expression plasmid, N-HA tag||HG14415-NY|
|Human CKB natural ORF mammalian expression plasmid||HG14415-UT|
|Learn more about expression Vectors|
CKB(Creatine kinase B type) contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. It belongs to the ATP:guanido phosphotransferase family. CKB consists of a homodimer of two identical brain-type CK-B subunits. CKB is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CKB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. CKB reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer in brain, smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone etc.