|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Human SNAP25 ORF mammalian expression plasmid, C-GFPSpark tag||HG11585-ACG|
|Human SNAP25 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11585-ACR|
|Human SNAP25 ORF mammalian expression plasmid, N-GFPSpark tag||HG11585-ANG|
|Human SNAP25 ORF mammalian expression plasmid, C-Flag tag||HG11585-CF|
|Human SNAP25 ORF mammalian expression plasmid, C-His tag||HG11585-CH|
|Human SNAP25 ORF mammalian expression plasmid, C-Myc tag||HG11585-CM|
|Human SNAP25 ORF mammalian expression plasmid, C-HA tag||HG11585-CY|
|Human SNAP25 Gene cDNA clone plasmid||HG11585-M|
|Human SNAP25 ORF mammalian expression plasmid, N-Flag tag||HG11585-NF|
|Human SNAP25 ORF mammalian expression plasmid, N-His tag||HG11585-NH|
|Human SNAP25 ORF mammalian expression plasmid, N-Myc tag||HG11585-NM|
|Human SNAP25 ORF mammalian expression plasmid, N-HA tag||HG11585-NY|
|Human SNAP25 natural ORF mammalian expression plasmid||HG11585-UT|
|Learn more about expression Vectors|
Synaptosomal-associated protein 25, also known as Super protein, Synaptosomal-associated 25 kDa protein, SNAP25 and SNAP, is a cytoplasm and cell membrane protein which belongs to the SNAP-25 family. SNAP25 / SUP contains 2 t-SNARE coiled-coil homology domains. SNAP25 / SUP is a membrane bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 / SUP protein is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP25 / SUP is a Q-SNARE protein contributing two α-helices in the formation of the exocytotic fusion complex in neurons where it assembles with syntaxin-1 and synaptobrevin. SNAP25 / SUP is involved in the molecular regulation of neurotransmitter release. It may play an important role in the synaptic function of specific neuronal systems. SNAP25 / SUP associates with proteins involved in vesicle docking and membrane fusion. SNAP25 / SUP regulates plasma membrane recycling through its interaction with CENPF. SNAP25 / SUP inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP25 / SUP decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.