|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human COMMD9 ORF mammalian expression plasmid, C-GFPSpark tag||HG14570-ACG|
|Human COMMD9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14570-ACR|
|Human COMMD9 ORF mammalian expression plasmid, N-GFPSpark tag||HG14570-ANG|
|Human COMMD9 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14570-ANR|
|Human COMMD9 ORF mammalian expression plasmid, C-Flag tag||HG14570-CF|
|Human COMMD9 ORF mammalian expression plasmid, C-His tag||HG14570-CH|
|Human COMMD9 ORF mammalian expression plasmid, C-Myc tag||HG14570-CM|
|Human COMMD9 ORF mammalian expression plasmid, C-HA tag||HG14570-CY|
|Human COMMD9 Gene cDNA clone plasmid||HG14570-G|
|Human COMMD9 ORF mammalian expression plasmid, N-Flag tag||HG14570-NF|
|Human COMMD9 ORF mammalian expression plasmid, N-His tag||HG14570-NH|
|Human COMMD9 ORF mammalian expression plasmid, N-Myc tag||HG14570-NM|
|Human COMMD9 ORF mammalian expression plasmid, N-HA tag||HG14570-NY|
|Human COMMD9 natural ORF mammalian expression plasmid||HG14570-UT|
|Learn more about expression Vectors|
COMMD9 is a COMM domain-containing or COMMD protein. COMMD family is comprised of ten members which are widely conserved throughout evolution and share certain functional properties. They represent a recently discovered set of evolutionarily conserved factors characterized by the presence of a defining carboxy-terminal motif. COMMD protein functions in the control of the transcription factor NFkappaB. NFkappaB plays a critical role in a number of homeostatic processes in multicellular organisms, including the regulation of immunity and cell survival. COMMD proteins inhibit NFkappaB mediated gene expression, and recent mechanistic studies have revealed that COMMD1 controls the ubiquitination of NFkappaB subunits, an event linked to transcriptional termination. COMMD1 binds to a multimeric ubiquitin ligase containing Elongins B/C, Cul2 and SOCS1 (ECS( SOCS1)). In this complex, COMMD1 facilitates the binding of NFkappaB subunits to the ligase, thereby promoting their ubiquitination and degradation. Additional insights gained from these studies indicate that COMMD proteins likely play a broader role in cellular homeostasis through their participation in the ubiquitination pathway.