|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human IZUMO1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13520-ACG|
|Human IZUMO1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13520-ACR|
|Human IZUMO1 ORF mammalian expression plasmid, C-Flag tag||HG13520-CF|
|Human IZUMO1 ORF mammalian expression plasmid, C-His tag||HG13520-CH|
|Human IZUMO1 ORF mammalian expression plasmid, C-Myc tag||HG13520-CM|
|Human IZUMO1 ORF mammalian expression plasmid, C-HA tag||HG13520-CY|
|Human IZUMO1 Gene cDNA clone plasmid||HG13520-G|
|Human IZUMO1 ORF mammalian expression plasmid, N-Flag tag||HG13520-NF|
|Human IZUMO1 ORF mammalian expression plasmid, N-His tag||HG13520-NH|
|Human IZUMO1 ORF mammalian expression plasmid, N-Myc tag||HG13520-NM|
|Human IZUMO1 ORF mammalian expression plasmid, N-HA tag||HG13520-NY|
|Human IZUMO1 natural ORF mammalian expression plasmid||HG13520-UT|
|Learn more about expression Vectors|
Izumo is a sperm membrane protein which plays a key role in the fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N-terminal domain for which neither the functions nor homologous sequences are known. Up to now, there four members has an N-terminal domain with significant homology to the N-terminal domain of Izumo. We call this domain Izumo domain. The four proteins are Izumo 1, 2, 3, and 4. Izumo domain possesses the ability to form dimers, whereas the transmembrane domain or the cytoplasmic domain or both of Izumo 1 are required for the formation of multimers of higher order. Izumo 1-3 are transmembrane proteins expressed specifically in the testis, and Izumo 4 is a soluble protein expressed in the testis and in other tissues. Izumo 1, 3, and 4 formed protein complexes on sperm, Izumo 1 forming several larger complexes and Izumo 3 and 4 forming a single larger complex. Izumo1 is essential for sperm-egg plasma membrane binding and fusion.