|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SELM ORF mammalian expression plasmid, C-GFPSpark tag||HG13508-ACG|
|Human SELM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13508-ACR|
|Human SELM ORF mammalian expression plasmid, N-GFPSpark tag||HG13508-ANG|
|Human SELM ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13508-ANR|
|Human SELM ORF mammalian expression plasmid, C-Flag tag||HG13508-CF|
|Human SELM ORF mammalian expression plasmid, C-His tag||HG13508-CH|
|Human SELM ORF mammalian expression plasmid, C-Myc tag||HG13508-CM|
|Human SELM ORF mammalian expression plasmid, C-HA tag||HG13508-CY|
|Human SELM Gene cDNA clone plasmid||HG13508-G|
|Human SELM ORF mammalian expression plasmid, N-Flag tag||HG13508-NF|
|Human SELM ORF mammalian expression plasmid, N-His tag||HG13508-NH|
|Human SELM ORF mammalian expression plasmid, N-Myc tag||HG13508-NM|
|Human SELM ORF mammalian expression plasmid, N-HA tag||HG13508-NY|
|Human SELM natural ORF mammalian expression plasmid||HG13508-UT|
|Learn more about expression Vectors|
Selenoprotein M is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine M is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This gene is expressed in a variety of tissues, and the protein is localized to the perinuclear structures. Selenoprotein M May function as a thiol-disulfide oxidoreductase that participates in disulfide bond formation. This protein is widely expressed and is highly expressed in brain. It is found in Cytoplasm, perinuclear region, Endoplasmic reticulum, Golgi apparatus. Localized to perinuclear structures corresponding to Golgi and endoplasmic reticulum. Experiments results have suggested that selenoprotein M may have an important role in protecting against oxidative damage in the brain and may potentially function in calcium regulation.