|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human DCX ORF mammalian expression plasmid, C-GFPSpark tag||HG13498-ACG|
|Human DCX ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13498-ACR|
|Human DCX ORF mammalian expression plasmid, N-GFPSpark tag||HG13498-ANG|
|Human DCX ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13498-ANR|
|Human DCX ORF mammalian expression plasmid, C-Flag tag||HG13498-CF|
|Human DCX ORF mammalian expression plasmid, C-His tag||HG13498-CH|
|Human DCX ORF mammalian expression plasmid, C-Myc tag||HG13498-CM|
|Human DCX ORF mammalian expression plasmid, C-HA tag||HG13498-CY|
|Human DCX Gene cDNA clone plasmid||HG13498-G|
|Human DCX ORF mammalian expression plasmid, N-Flag tag||HG13498-NF|
|Human DCX ORF mammalian expression plasmid, N-His tag||HG13498-NH|
|Human DCX ORF mammalian expression plasmid, N-Myc tag||HG13498-NM|
|Human DCX ORF mammalian expression plasmid, N-HA tag||HG13498-NY|
|Human DCX natural ORF mammalian expression plasmid||HG13498-UT|
|Learn more about expression Vectors|
DCX (doublecortin, N-GST chimera)contains 2 doublecortin domains and belongs to the doublecortin family. It is highly expressed in neuronal cells of fetal brain, but not expressed in other fetal tissues. In the adult, it is highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. DCX is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. DCX may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. It may be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Defects in DCX are the cause of lissencephaly X-linked type 1 and subcortical band heterotopia X-linked.