|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PTS ORF mammalian expression plasmid, C-GFPSpark tag||HG14332-ACG|
|Human PTS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14332-ACR|
|Human PTS ORF mammalian expression plasmid, N-GFPSpark tag||HG14332-ANG|
|Human PTS ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14332-ANR|
|Human PTS ORF mammalian expression plasmid, C-Flag tag||HG14332-CF|
|Human PTS ORF mammalian expression plasmid, C-His tag||HG14332-CH|
|Human PTS ORF mammalian expression plasmid, C-Myc tag||HG14332-CM|
|Human PTS ORF mammalian expression plasmid, C-HA tag||HG14332-CY|
|Human PTS Gene cDNA clone plasmid||HG14332-G|
|Human PTS ORF mammalian expression plasmid, N-Flag tag||HG14332-NF|
|Human PTS ORF mammalian expression plasmid, N-His tag||HG14332-NH|
|Human PTS ORF mammalian expression plasmid, N-Myc tag||HG14332-NM|
|Human PTS ORF mammalian expression plasmid, N-HA tag||HG14332-NY|
|Human PTS natural ORF mammalian expression plasmid||HG14332-UT|
|Learn more about expression Vectors|
PTS(6-pyruvoyltetrahydropterin synthase) belongs to the PTPS family. It catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. PTS is involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. PTS also catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin. Defects in PTS are the cause of BH4-deficient hyperphenylalaninemia type A (HPABH4A), also called 6-pyruvoyl-tetrahydropterin synthase deficiency (PTS deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to PTS deficiency. HPABH4A is an autosomal recessive disorder characterized by depletion of the neurotransmitters dopamine and serotonin, and clinically by severe neurological symptoms unresponsive to the classic phenylalanine-low diet.