|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ENSA ORF mammalian expression plasmid, C-GFPSpark tag||HG14321-ACG|
|Human ENSA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14321-ACR|
|Human ENSA ORF mammalian expression plasmid, N-GFPSpark tag||HG14321-ANG|
|Human ENSA ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14321-ANR|
|Human ENSA ORF mammalian expression plasmid, C-Flag tag||HG14321-CF|
|Human ENSA ORF mammalian expression plasmid, C-His tag||HG14321-CH|
|Human ENSA ORF mammalian expression plasmid, C-Myc tag||HG14321-CM|
|Human ENSA ORF mammalian expression plasmid, C-HA tag||HG14321-CY|
|Human ENSA Gene cDNA clone plasmid||HG14321-G|
|Human ENSA ORF mammalian expression plasmid, N-Flag tag||HG14321-NF|
|Human ENSA ORF mammalian expression plasmid, N-His tag||HG14321-NH|
|Human ENSA ORF mammalian expression plasmid, N-Myc tag||HG14321-NM|
|Human ENSA ORF mammalian expression plasmid, N-HA tag||HG14321-NY|
|Human ENSA natural ORF mammalian expression plasmid||HG14321-UT|
|Learn more about expression Vectors|
Endosulfine alpha, also known as ENSA, belongs to the endosulfine family. It is a highly conserved cAMP-regulated phosphoprotein (ARPP) family. Endosulfine alpha is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. As a protein phosphatase inhibitor, ENSA specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase By similarity. Endosulfine alpha also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.