|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Human ARSA ORF mammalian expression plasmid, C-GFPSpark tag||HG10449-ACG|
|Human ARSA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10449-ACR|
|Human ARSA ORF mammalian expression plasmid, C-Flag tag||HG10449-CF|
|Human ARSA ORF mammalian expression plasmid, C-His tag||HG10449-CH|
|Human ARSA ORF mammalian expression plasmid, C-Myc tag||HG10449-CM|
|Human ARSA ORF mammalian expression plasmid, C-HA tag||HG10449-CY|
|Human ARSA Gene cDNA clone plasmid||HG10449-M|
|Human ARSA ORF mammalian expression plasmid, Flag tag||HG10449-M-F|
|Human ARSA natural ORF mammalian expression plasmid||HG10449-M-N|
|Human ARSA ORF mammalian expression plasmid, N-Flag tag||HG10449-NF|
|Human ARSA ORF mammalian expression plasmid, N-His tag||HG10449-NH|
|Human ARSA ORF mammalian expression plasmid, N-Myc tag||HG10449-NM|
|Human ARSA ORF mammalian expression plasmid, N-HA tag||HG10449-NY|
|Human ARSA natural ORF mammalian expression plasmid||HG10449-UT|
|Learn more about expression Vectors|
Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH-dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system. Additionally, the serum level of arylsulfatase A might be helpful in diagnosis of lung and central nervous system cancer.