|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human NAA10 ORF mammalian expression plasmid, C-GFPSpark tag||HG14293-ACG|
|Human NAA10 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14293-ACR|
|Human NAA10 ORF mammalian expression plasmid, N-GFPSpark tag||HG14293-ANG|
|Human NAA10 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14293-ANR|
|Human NAA10 ORF mammalian expression plasmid, C-Flag tag||HG14293-CF|
|Human NAA10 ORF mammalian expression plasmid, C-His tag||HG14293-CH|
|Human NAA10 ORF mammalian expression plasmid, C-Myc tag||HG14293-CM|
|Human NAA10 ORF mammalian expression plasmid, C-HA tag||HG14293-CY|
|Human NAA10 Gene cDNA clone plasmid||HG14293-G|
|Human NAA10 ORF mammalian expression plasmid, N-Flag tag||HG14293-NF|
|Human NAA10 ORF mammalian expression plasmid, N-His tag||HG14293-NH|
|Human NAA10 ORF mammalian expression plasmid, N-Myc tag||HG14293-NM|
|Human NAA10 ORF mammalian expression plasmid, N-HA tag||HG14293-NY|
|Human NAA10 natural ORF mammalian expression plasmid||HG14293-UT|
|Learn more about expression Vectors|
ARD1 is a member of the 20-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 403-574), and a 46-kDa N-terminal domain (amino acids 1-402). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating type locus HML, for sporulation, and for entry into G0. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-110) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.