|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SCG2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13441-ACG|
|Human SCG2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13441-ACR|
|Human SCG2 ORF mammalian expression plasmid, C-Flag tag||HG13441-CF|
|Human SCG2 ORF mammalian expression plasmid, C-His tag||HG13441-CH|
|Human SCG2 ORF mammalian expression plasmid, C-Myc tag||HG13441-CM|
|Human SCG2 ORF mammalian expression plasmid, C-HA tag||HG13441-CY|
|Human SCG2 Gene cDNA clone plasmid||HG13441-G|
|Human SCG2 ORF mammalian expression plasmid, N-Flag tag||HG13441-NF|
|Human SCG2 ORF mammalian expression plasmid, N-His tag||HG13441-NH|
|Human SCG2 ORF mammalian expression plasmid, N-Myc tag||HG13441-NM|
|Human SCG2 ORF mammalian expression plasmid, N-HA tag||HG13441-NY|
|Human SCG2 natural ORF mammalian expression plasmid||HG13441-UT|
|Learn more about expression Vectors|
Kit ligand, also known as Hematopoietic growth factor KL, Mast cell growth factor, Steel factor, Stem cell factor, c-Kit ligand, Kitlg and KITL, is a single-pass type I membrane protein which belongs to the SCF family. KITL / kit ligand also belongs to the family of dimeric transmembrane growth factors. The soluble form of KIT ligand is a secreted protein. Mast cells are thought to participate in a variety of immune responses, such as parasite resistance and the allergic reaction. Mast cell development depends on stem cell factor (Kit ligand) and its receptor, c-Kit. KITL / kit ligand stimulates the proliferation of mast cells. KITL / kit ligand is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Efficient cell surface presentation of KITL / kit ligand is essential for the migration, proliferation, and survival of melanocytes, germ cells, hemopoietic stem cells, and mastocytes. KITL / kit ligand acts synergistically with other cytokines, probably interleukins. KITL / kit ligand plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. It exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes. KITL / kit ligand misexpression in some hyperpigmented lesions may open the avenue for Kitl-dependent treatment of pathological skin conditions.