|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ABHD10 ORF mammalian expression plasmid, C-GFPSpark tag||HG14439-ACG|
|Human ABHD10 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14439-ACR|
|Human ABHD10 ORF mammalian expression plasmid, N-GFPSpark tag||HG14439-ANG|
|Human ABHD10 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14439-ANR|
|Human ABHD10 ORF mammalian expression plasmid, C-Flag tag||HG14439-CF|
|Human ABHD10 ORF mammalian expression plasmid, C-His tag||HG14439-CH|
|Human ABHD10 ORF mammalian expression plasmid, C-Myc tag||HG14439-CM|
|Human ABHD10 ORF mammalian expression plasmid, C-HA tag||HG14439-CY|
|Human ABHD10 Gene cDNA clone plasmid||HG14439-G|
|Human ABHD10 ORF mammalian expression plasmid, N-Flag tag||HG14439-NF|
|Human ABHD10 ORF mammalian expression plasmid, N-His tag||HG14439-NH|
|Human ABHD10 ORF mammalian expression plasmid, N-Myc tag||HG14439-NM|
|Human ABHD10 ORF mammalian expression plasmid, N-HA tag||HG14439-NY|
|Human ABHD10 natural ORF mammalian expression plasmid||HG14439-UT|
|Learn more about expression Vectors|
Mycophenolic acid (MPA), the active metabolite of the immunosuppressant mycophenolate mofetil (MMF), is primarily metabolized by glucuronidation to a phenolic glucuronide (MPAG) and an acyl glucuronide (AcMPAG). It is known that AcMPAG, which may be an immunotoxic metabolite, is deglucuronidated in human liver. AcMPAG deglucuronidation activity was detected in both human liver cytosol (HLC) and microsomes (HLM). By purification from HLC with column chromatographic purification steps, the enzyme responsible for AcMPAG deglucuronidationis identified as α/β hydrolase domain containing 10 (ABHD10). Recombinant ABHD10 expressed in Sf9 cells efficiently deglucuronidated AcMPAG with a K(m) value of 100.7 ± 10.2 μM, which was similar to those in HLM, HLC, and human liver homogenates (HLH). Immunoblot analysis revealed ABHD10 protein expression in both HLC and HLM. The AcMPAG deglucuronidation by recombinant ABHD10, HLC, and HLH were potently inhibited by AgNO(3), CdCl(2), CuCl(2), PMSF, bis-p-nitrophenylphosphate, and DTNB. The CL(int) value of AcMPAG formation from MPA, which was catalyzed by human UGT2B7, in HLH was increased by 1.8-fold in the presence of PMSF. Thus, human ABHD10 would affect the formation of AcMPAG, the immunotoxic metabolite.