|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human STX8 ORF mammalian expression plasmid, C-GFPSpark tag||HG14243-ACG|
|Human STX8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14243-ACR|
|Human STX8 ORF mammalian expression plasmid, N-GFPSpark tag||HG14243-ANG|
|Human STX8 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14243-ANR|
|Human STX8 ORF mammalian expression plasmid, C-Flag tag||HG14243-CF|
|Human STX8 ORF mammalian expression plasmid, C-His tag||HG14243-CH|
|Human STX8 ORF mammalian expression plasmid, C-Myc tag||HG14243-CM|
|Human STX8 ORF mammalian expression plasmid, C-HA tag||HG14243-CY|
|Human STX8 Gene cDNA clone plasmid||HG14243-G|
|Human STX8 ORF mammalian expression plasmid, N-Flag tag||HG14243-NF|
|Human STX8 ORF mammalian expression plasmid, N-His tag||HG14243-NH|
|Human STX8 ORF mammalian expression plasmid, N-Myc tag||HG14243-NM|
|Human STX8 ORF mammalian expression plasmid, N-HA tag||HG14243-NY|
|Human STX8 natural ORF mammalian expression plasmid||HG14243-UT|
|Learn more about expression Vectors|
STX8, also known as syntaxin 8, directly interacts with HECTd3. STX8 forms the SNARE complex with syntaxin 7, vti1b and endobrevin. STX8 belongs to the syntaxin family. Members of this family are key molecules implicated in diverse vesicle docking and membrane fusion events. STX8 physically interacts with cystic fibrosis transmembrane conductance regulator (CFTR): recombinant syntaxin 8 binds CFTR in vitro and both proteins co-immunoprecipitate in HT29 cells. Syntaxin 8 regulates CFTR-mediated currents in chinese hamster ovary (CHO) cells stably expressing CFTR and syntaxin 8. STX8 contributes to the regulation of CFTR trafficking and chloride channel activity by the SNARE machinery.