|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Human HGFA ORF mammalian expression plasmid, C-GFPSpark tag||HG10329-ACG|
|Human HGFA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10329-ACR|
|Human HGFA ORF mammalian expression plasmid, C-Flag tag||HG10329-CF|
|Human HGFA ORF mammalian expression plasmid, C-His tag||HG10329-CH|
|Human HGFA ORF mammalian expression plasmid, C-Myc tag||HG10329-CM|
|Human HGFA ORF mammalian expression plasmid, C-HA tag||HG10329-CY|
|Human HGFA Gene cDNA clone plasmid||HG10329-M|
|Human HGFA ORF mammalian expression plasmid, N-Flag tag||HG10329-NF|
|Human HGFA ORF mammalian expression plasmid, N-His tag||HG10329-NH|
|Human HGFA ORF mammalian expression plasmid, N-Myc tag||HG10329-NM|
|Human HGFA ORF mammalian expression plasmid, N-HA tag||HG10329-NY|
|Human HGFA natural ORF mammalian expression plasmid||HG10329-UT|
|Learn more about expression Vectors|
HGF activator (HGFA) is a serum-derived serine protease and belongs to the peptidase family S1.HGFA is responsible for the conversion of hepatocyte growth factor (HGF), from the inactive single-chain precursor to the active heterodimeric form, which is a potent mitogen, motogen, and morphogen for liver cells, epithelial cells, and endothelial cells. HGFA is synthesized and secreted by the liver and circulates in the plasma as an inactive single-chain zymogen in normal states. The zymogen is cleaved by thrombin or thermolysin through the endoproteolytic process and forms an active heterodimer linked by a disulfide bond. In turn, the active protease can be inhibited by HGFA inhibitors (HAIs) including HAI-1 and HAI-2. In addition, the HGFA zymogen acquires a strong affinity upon activation and thus may ensure the local action in tissue regeneration in liver, kidney and skin. It has been reported that activation of HGF is a critical limiting step in the HGF/SF-induced signaling pathway mediated by Met, and accordingly, aberrant expression of HGFA is implicated in tumorigenesis and progression.
1. Shimomura, T. et al., 1993, J. Biol. Chem. 268: 22927-22932.
2. Miyazawa, K. et al., 1996, J. Biol. Chem. 271 : 3615-3618.
3. Shia, S. et al., 2005, J. Mol. Biol. 346: 1335-1349.
4. Kataoka, H. et al., 2000, J. Biol. Chem. 275: 40453-40462.
5. Tjin, E.P. et al., 2006, Blood. 107: 760-768.
6. Kitajima, Y. et al., 2000, Cancer. Res. 60: 6148-6159.