|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human PROCR ORF mammalian expression plasmid, C-GFPSpark tag||HG13320-ACG|
|Human PROCR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13320-ACR|
|Human PROCR ORF mammalian expression plasmid, C-Flag tag||HG13320-CF|
|Human PROCR ORF mammalian expression plasmid, C-His tag||HG13320-CH|
|Human PROCR ORF mammalian expression plasmid, C-Myc tag||HG13320-CM|
|Human PROCR ORF mammalian expression plasmid, C-HA tag||HG13320-CY|
|Human PROCR Gene cDNA clone plasmid||HG13320-G|
|Human PROCR ORF mammalian expression plasmid, N-Flag tag||HG13320-NF|
|Human PROCR ORF mammalian expression plasmid, N-His tag||HG13320-NH|
|Human PROCR ORF mammalian expression plasmid, N-Myc tag||HG13320-NM|
|Human PROCR ORF mammalian expression plasmid, N-HA tag||HG13320-NY|
|Human PROCR natural ORF mammalian expression plasmid||HG13320-UT|
|Learn more about expression Vectors|
Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor) or PROCR, is a receptor for Protein C. Protein C plays an important role in many metabolism processes in humans and other animals after activated by binding to Endothelial protein C receptor (EPCR). Because of the EPCR is found primarily on endothelial cells (cells on the inside of blood vessels), activated protein C is found maily near endothelial cells. Protein C is pleiotropic, with two main functions: anticoagulation and cytoprotection. Which function will be performed depend on whether or not protein C remains bind to EPCR after activated. The anticoagulation occurs when it does not. In this case, protein C functions as an anticoagulant by irreversibly proteolytically inactivating Factor Va and Factor VIIIa, turning them into Factor Vi and Factor VIIIi respectively. When still bound to EPCR, activated protein C performs its cytoprotective effects, acting on the effector substrate PAR-1, protease-activated receptor-1. To a degree, APC's anticoagulant properties are independent of its cytoprotective ones, in that expression of one pathway is not affected by the existence of the other.