|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IFITM3 ORF mammalian expression plasmid, C-GFPSpark tag||HG14141-ACG|
|Human IFITM3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14141-ACR|
|Human IFITM3 ORF mammalian expression plasmid, C-Flag tag||HG14141-CF|
|Human IFITM3 ORF mammalian expression plasmid, C-His tag||HG14141-CH|
|Human IFITM3 ORF mammalian expression plasmid, C-Myc tag||HG14141-CM|
|Human IFITM3 ORF mammalian expression plasmid, C-HA tag||HG14141-CY|
|Human IFITM3 Gene cDNA clone plasmid||HG14141-G|
|Human IFITM3 ORF mammalian expression plasmid, N-Flag tag||HG14141-NF|
|Human IFITM3 ORF mammalian expression plasmid, N-His tag||HG14141-NH|
|Human IFITM3 ORF mammalian expression plasmid, N-Myc tag||HG14141-NM|
|Human IFITM3 ORF mammalian expression plasmid, N-HA tag||HG14141-NY|
|Human IFITM3 natural ORF mammalian expression plasmid||HG14141-UT|
|Learn more about expression Vectors|
Interferon-induced transmembrane protein 3 (IFITM3) belongs to the CD225 family. To replicate, viruses must gain access to the host cell's resources. Interferon (IFN) regulates the actions of a large complement of interferon effector genes (IEGs) that prevent viral replication. The interferon inducible transmembrane protein family members, IFITM1, 2 and 3, are IEGs required for inhibition of influenza A virus, dengue virus, and West Nile virus replication in vitro. IFITM3 is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. It is both necessary and sufficient for preventing the emergence of viral genomes from the endosomal pathway. Viral pseudoparticles were inhibited from transferring their contents into the host cell cytosol by IFN, and IFITM3 was required and sufficient for this action. IFITM3 overexpression is sufficient for this phenotype. Moreover, IFITM3 partially resides in late endosomal and lysosomal structures, placing it in the path of invading viruses.