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Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-tagged

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PRSS8cDNA Clone Product Information
Gene_bank_ref_id:NM_002773.3
cDNA Size:1032
cDNA Description:ORF Clone of Homo sapiens protease, serine, 8 DNA.
Gene Synonym:CAP1, PROSTASIN
Species:Human
Vector:pCMV3-N-His
Restriction Site:
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-SP-N-His (suitable for secretary and membane protein expession) Vector Information
 
Vector Name pCMV3-SP-N-His
Vector Size 6149bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag His
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-SP-N-His (suitable for secretary and membane protein expession) Physical Map
Schematic of pCMV3-SP-N-His (suitable for secretary and membane protein expession) Multiple Cloning Sites

His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-tagged on other vectors
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10287-ACG$325
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10287-ACR$325
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10287-CF$295
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10287-CH$295
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10287-CM$295
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10287-CY$295
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone)HG10287-M$95
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone) expression ready, FLAG-taggedHG10287-M-F$295
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone) expression ready, untaggedHG10287-M-N$295
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10287-NF$295
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10287-NH$295
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10287-NM$295
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10287-NY$295
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10287-UT$295
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Background

Prostasin (Prss8), also known as channel activating protease 1 (CAP1), is a trypsinlike serine peptidase, and plays important roles in epithelial physiology. It is originally purified as an active, soluble enzyme from human seminal fluid and is highly expressed in prostate, lung, kidney, salivary gland and pancreas. Prostasin is expressed as a glycosyl-phosphatidylinositol (GPI)-anchored membrane protein in prostate epithelial cells, and also exists as a secreted proteolytic enzyme possibly via tryptic cleavage of its COOH-terminal hydrophobic domain. Prostasin is found to activate the epithelial sodium channel (ENaC) which is tightly regulated and is critical for maintaining salt and fluid balance in the lung and kidney in both normal and pathological conditions. Accordingly, prostasin has been proposed as a target for therapeutic inhibition in cystic fibrosis. In addition, prostasin inhibits prostate and breast cancer cell invasion in vitro, suggesting a functional role as a suppressor of tumor invasion, as well as a regulator of gene expression during inflammation.

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