|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Human MIF ORF mammalian expression plasmid, C-GFPSpark tag||HG10246-ACG|
|Human MIF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10246-ACR|
|Human MIF ORF mammalian expression plasmid, C-Flag tag||HG10246-CF|
|Human MIF ORF mammalian expression plasmid, C-His tag||HG10246-CH|
|Human MIF ORF mammalian expression plasmid, C-Myc tag||HG10246-CM|
|Human MIF ORF mammalian expression plasmid, C-HA tag||HG10246-CY|
|Human MIF Gene cDNA clone plasmid||HG10246-M|
|Human MIF ORF mammalian expression plasmid, N-Flag tag||HG10246-NF|
|Human MIF ORF mammalian expression plasmid, N-His tag||HG10246-NH|
|Human MIF ORF mammalian expression plasmid, N-Myc tag||HG10246-NM|
|Human MIF ORF mammalian expression plasmid, N-HA tag||HG10246-NY|
|Human MIF natural ORF mammalian expression plasmid||HG10246-UT|
|Learn more about expression Vectors|
Macrophage migration inhibitory factor (MIF) is an immunoregulatory cytokine, the effect of which on arresting random immune cell movement was recognized several decades ago. Despite its historic name, MIF also has a direct chemokine-like function and promotes cell recruitment. MIF is an ubiquitously expressed protein that plays a crucial role in many inflammatory and autoimmune disorders. Increasing evidence suggests that MIF also controls metabolic and inflammatory processes underlying the development of metabolic pathologies associated with obesity. Further research has shown that MIF plays a particularly critical part in cell cycle regulation and therefore in tumorigenesis as well. The significance of the role of MIF in a variety of both solid and hematologic tumors has been established. More recently, interest has increased in the role of MIF in the development of central nervous system (CNS) tumors, in which it appears to influence cell cycle control. MIF contributes to malignant disease progression on several different levels. Both circulating and intracellular MIF protein levels are elevated in cancer patients and MIF expression reportedly correlates with stage, metastatic spread and disease-free survival. Blockade of MIF bioactivity successfully inhibited tumor cell growth in vivo and in vitro. MIF plays important roles in the pathogenesis of gastrointestinal, hepatic, and pancreatic disorders.