|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human PDGFD ORF mammalian expression plasmid, C-GFPSpark tag||HG13232-ACG|
|Human PDGFD ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13232-ACR|
|Human PDGFD ORF mammalian expression plasmid, C-Flag tag||HG13232-CF|
|Human PDGFD ORF mammalian expression plasmid, C-His tag||HG13232-CH|
|Human PDGFD ORF mammalian expression plasmid, C-Myc tag||HG13232-CM|
|Human PDGFD ORF mammalian expression plasmid, C-HA tag||HG13232-CY|
|Human PDGFD Gene cDNA clone plasmid||HG13232-G|
|Human PDGFD ORF mammalian expression plasmid, N-Flag tag||HG13232-NF|
|Human PDGFD ORF mammalian expression plasmid, N-His tag||HG13232-NH|
|Human PDGFD ORF mammalian expression plasmid, N-Myc tag||HG13232-NM|
|Human PDGFD ORF mammalian expression plasmid, N-HA tag||HG13232-NY|
|Human PDGFD natural ORF mammalian expression plasmid||HG13232-UT|
|Learn more about expression Vectors|
Platelet-derived growth factor D (PDGF-D), also known as Iris-expressed growth factor, is a member of the PDGF/vascular endothelial growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. PDGF-D/PDGFD only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. The expression of PDGF-D/PDGFD in the eye is tissue-specific. In the anterior segment, it is localized to iris and ciliary body, whereas in the retina, PDGF-D/PDGFD is restricted to the outer plexiform layer. PDGF-D/PDGFD is present in aqueous humor but is not detectable in mature lens or in mouse lens-derived alphaTN4-1 cells. PDGF-D/PDGFD is highly expressed in human breast cancer and facilitates tumor growth and lymph node metastasis, making it a potential target in breast cancer. PDGF-D/PDGFD increases drug delivery and hence improves the efficacy of chemotherapy through vessel normalization. Intervention in the PDGF-D pathway in the eye, perhaps by antibody or blocking peptide, could be useful in the treatment of certain cataracts, including post-operative secondary cataract.