|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human PTPRJ ORF mammalian expression plasmid, C-GFPSpark tag||HG13165-ACG|
|Human PTPRJ ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13165-ACR|
|Human PTPRJ ORF mammalian expression plasmid, C-Flag tag||HG13165-CF|
|Human PTPRJ ORF mammalian expression plasmid, C-His tag||HG13165-CH|
|Human PTPRJ ORF mammalian expression plasmid, C-Myc tag||HG13165-CM|
|Human PTPRJ ORF mammalian expression plasmid, C-HA tag||HG13165-CY|
|Human PTPRJ Gene cDNA clone plasmid||HG13165-G|
|Human PTPRJ ORF mammalian expression plasmid, N-Flag tag||HG13165-NF|
|Human PTPRJ ORF mammalian expression plasmid, N-His tag||HG13165-NH|
|Human PTPRJ ORF mammalian expression plasmid, N-Myc tag||HG13165-NM|
|Human PTPRJ ORF mammalian expression plasmid, N-HA tag||HG13165-NY|
|Human PTPRJ natural ORF mammalian expression plasmid||HG13165-UT|
|Learn more about expression Vectors|
DEP1 / PTPRJ (Receptor-type tyrosine-protein phosphatase eta) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. DEP1 / PTPRJ possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. DEP1 / PTPRJ is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. In stable MCF-7 cell lines lines, induction of DEP-1 expression inhibited breast cancer cell growth by 5-10-fold. These data describe PTPs expressed and regulated in breast cancer cell lines during differentiation and identify one PTP, DEP-1, that inhibits the growth of breast cancer cells in vitro.