|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MMGT1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13998-ACG|
|Human MMGT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13998-ACR|
|Human MMGT1 ORF mammalian expression plasmid, C-Flag tag||HG13998-CF|
|Human MMGT1 ORF mammalian expression plasmid, C-His tag||HG13998-CH|
|Human MMGT1 ORF mammalian expression plasmid, C-Myc tag||HG13998-CM|
|Human MMGT1 ORF mammalian expression plasmid, C-HA tag||HG13998-CY|
|Human MMGT1 Gene cDNA clone plasmid||HG13998-G|
|Human MMGT1 ORF mammalian expression plasmid, N-Flag tag||HG13998-NF|
|Human MMGT1 ORF mammalian expression plasmid, N-His tag||HG13998-NH|
|Human MMGT1 ORF mammalian expression plasmid, N-Myc tag||HG13998-NM|
|Human MMGT1 ORF mammalian expression plasmid, N-HA tag||HG13998-NY|
|Human MMGT1 natural ORF mammalian expression plasmid||HG13998-UT|
|Learn more about expression Vectors|
MMGT1, also known as EMC5, is comparable with primary microglial cells with respect to morphology, presence of acetylated low density lipoprotein receptor, non-specific esterase, CD63, major histocompatibility complex antigens and CD11, and binding for Ricinus communis agglutinin. Primary microglia as well as MMGT1 cells are negative for glial fibrillary acidic protein. Different MMGT1 strains are obtained after subcloning, two of which resembled histiocytes (F4/80 and BM-8). These cell strains, MMGT12 and 16, are able to opsonize latex beads, and could be induced by endotoxins (LPS) to secrete TNF-alpha, IL-1, IL-6, TGF-beta, and EGF.