|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human MSLN ORF mammalian expression plasmid, C-GFPSpark tag||HG13128-ACG|
|Human MSLN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13128-ACR|
|Human MSLN ORF mammalian expression plasmid, C-Flag tag||HG13128-CF|
|Human MSLN ORF mammalian expression plasmid, C-His tag||HG13128-CH|
|Human MSLN ORF mammalian expression plasmid, C-Myc tag||HG13128-CM|
|Human MSLN ORF mammalian expression plasmid, C-HA tag||HG13128-CY|
|Human MSLN Gene cDNA clone plasmid||HG13128-G|
|Human MSLN natural ORF mammalian expression plasmid||HG13128-G-N|
|Human MSLN ORF mammalian expression plasmid, N-Flag tag||HG13128-NF|
|Human MSLN ORF mammalian expression plasmid, N-His tag||HG13128-NH|
|Human MSLN ORF mammalian expression plasmid, N-Myc tag||HG13128-NM|
|Human MSLN ORF mammalian expression plasmid, N-HA tag||HG13128-NY|
|Human MSLN natural ORF mammalian expression plasmid||HG13128-UT|
|Learn more about expression Vectors|
Megakaryocyte potentiating factor belongs to the mesothelin family. This family is comprised by several mammalian pre-pro-megakaryocyte potentiating factor precursor (MPF) or mesothelin proteins. Mesothelin is a glycosylphosphatidylinositol-linked glycoprotein highly expressed in mesothelial cells, mesotheliomas, and ovarian cancer, but the biological function of the protein is not known. Megakaryocyte potentiating factor is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). It interacts with MUC16 and potentiates megakaryocyte colony formation in vitro. Megakaryocyte potentiating factor is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment.