|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human VASN ORF mammalian expression plasmid, C-GFPSpark tag||HG13854-ACG|
|Human VASN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13854-ACR|
|Human VASN ORF mammalian expression plasmid, C-Flag tag||HG13854-CF|
|Human VASN ORF mammalian expression plasmid, C-His tag||HG13854-CH|
|Human VASN ORF mammalian expression plasmid, C-Myc tag||HG13854-CM|
|Human VASN ORF mammalian expression plasmid, C-HA tag||HG13854-CY|
|Human VASN Gene cDNA clone plasmid||HG13854-G|
|Human VASN ORF mammalian expression plasmid, N-Flag tag||HG13854-NF|
|Human VASN ORF mammalian expression plasmid, N-His tag||HG13854-NH|
|Human VASN ORF mammalian expression plasmid, N-Myc tag||HG13854-NM|
|Human VASN ORF mammalian expression plasmid, N-HA tag||HG13854-NY|
|Human VASN natural ORF mammalian expression plasmid||HG13854-UT|
|Learn more about expression Vectors|
Vasorin is a typical type I membrane protein. It contains 1 EGF-like domain, 1 fibronectin type-III domain, 10 LRR (leucine-rich) repeats, 1 LRRCT domain and 1 LRRNT domain. Vasorin is predominantly expressed in vascular smooth muscle cells, and that its expression is developmentally regulated. vasorin It directly binds to transforming growth factor (TGF)-β and attenuates TGF-β signaling in vitro. This suggests that down-regulation of vasorin expression contributes to neointimal formation after vascular injury and that vasorin modulates cellular responses to pathological stimuli in the vessel wall.