|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human C7 ORF mammalian expression plasmid, C-GFPSpark tag||HG13848-ACG|
|Human C7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13848-ACR|
|Human C7 ORF mammalian expression plasmid, C-Flag tag||HG13848-CF|
|Human C7 ORF mammalian expression plasmid, C-His tag||HG13848-CH|
|Human C7 ORF mammalian expression plasmid, C-Myc tag||HG13848-CM|
|Human C7 ORF mammalian expression plasmid, C-HA tag||HG13848-CY|
|Human C7 Gene cDNA clone plasmid||HG13848-G|
|Human C7 ORF mammalian expression plasmid, N-Flag tag||HG13848-NF|
|Human C7 ORF mammalian expression plasmid, N-His tag||HG13848-NH|
|Human C7 ORF mammalian expression plasmid, N-Myc tag||HG13848-NM|
|Human C7 ORF mammalian expression plasmid, N-HA tag||HG13848-NY|
|Human C7 natural ORF mammalian expression plasmid||HG13848-UT|
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Complement component 7 is a component of the complement system. It belongs to the complement C6/C7/C8/C9 family. It contains 1 EGF-like domain, 1 LDL-receptor class A domain, 1 MACPF domain, 2 Sushi (CCP/SCR) domains and 2 TSP type-1 domains. Complement component 7 serves as a membrane anchor. It participates in the formation of Membrane Attack Complex (MAC). People with C7 deficiency are prone to bacterial infection. It is a constituent of MAC that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Defects in C7 are a cause of complement component 7 deficiency (C7D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.