|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human UBASH3A ORF mammalian expression plasmid, C-GFPSpark tag||HG13847-ACG|
|Human UBASH3A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13847-ACR|
|Human UBASH3A ORF mammalian expression plasmid, N-GFPSpark tag||HG13847-ANG|
|Human UBASH3A ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13847-ANR|
|Human UBASH3A ORF mammalian expression plasmid, C-Flag tag||HG13847-CF|
|Human UBASH3A ORF mammalian expression plasmid, C-His tag||HG13847-CH|
|Human UBASH3A ORF mammalian expression plasmid, C-Myc tag||HG13847-CM|
|Human UBASH3A ORF mammalian expression plasmid, C-HA tag||HG13847-CY|
|Human UBASH3A Gene cDNA clone plasmid||HG13847-G|
|Human UBASH3A ORF mammalian expression plasmid, N-Flag tag||HG13847-NF|
|Human UBASH3A ORF mammalian expression plasmid, N-His tag||HG13847-NH|
|Human UBASH3A ORF mammalian expression plasmid, N-Myc tag||HG13847-NM|
|Human UBASH3A ORF mammalian expression plasmid, N-HA tag||HG13847-NY|
|Human UBASH3A natural ORF mammalian expression plasmid||HG13847-UT|
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UBASH3A is a member of the T-cell ubiquitin ligand (TULA) family. This family consists of two members. Both of them can negatively regulate T-cell signaling. UBASH3A can facilitate growth factor withdrawal-induced apoptosis in T cells, which may occur via its interaction with AIF, an apoptosis-inducing factor. Alternative splicing of UBASH3A gene results in multiple transcript variants. It interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. UBASH3A promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. UBASH3A also exhibits negligigle protein tyrosine phosphatase activity at neutral pH. It may act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. It may also inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.