|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human EBAG9 ORF mammalian expression plasmid, C-GFPSpark tag||HG14012-ACG|
|Human EBAG9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14012-ACR|
|Human EBAG9 ORF mammalian expression plasmid, C-Flag tag||HG14012-CF|
|Human EBAG9 ORF mammalian expression plasmid, C-His tag||HG14012-CH|
|Human EBAG9 ORF mammalian expression plasmid, C-Myc tag||HG14012-CM|
|Human EBAG9 ORF mammalian expression plasmid, C-HA tag||HG14012-CY|
|Human EBAG9 Gene cDNA clone plasmid||HG14012-G|
|Human EBAG9 ORF mammalian expression plasmid, N-Flag tag||HG14012-NF|
|Human EBAG9 ORF mammalian expression plasmid, N-His tag||HG14012-NH|
|Human EBAG9 ORF mammalian expression plasmid, N-Myc tag||HG14012-NM|
|Human EBAG9 ORF mammalian expression plasmid, N-HA tag||HG14012-NY|
|Human EBAG9 natural ORF mammalian expression plasmid||HG14012-UT|
|Learn more about expression Vectors|
RCAS1, also known as EBAG9, is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. RCAS1 gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of RCAS1 gene. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for RCAS1 gene. EBAG9 may participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.