|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LMAN2L ORF mammalian expression plasmid, C-GFPSpark tag||HG13822-ACG|
|Human LMAN2L ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13822-ACR|
|Human LMAN2L ORF mammalian expression plasmid, C-Flag tag||HG13822-CF|
|Human LMAN2L ORF mammalian expression plasmid, C-His tag||HG13822-CH|
|Human LMAN2L ORF mammalian expression plasmid, C-Myc tag||HG13822-CM|
|Human LMAN2L ORF mammalian expression plasmid, C-HA tag||HG13822-CY|
|Human LMAN2L Gene cDNA clone plasmid||HG13822-G|
|Human LMAN2L ORF mammalian expression plasmid, N-Flag tag||HG13822-NF|
|Human LMAN2L ORF mammalian expression plasmid, N-His tag||HG13822-NH|
|Human LMAN2L ORF mammalian expression plasmid, N-Myc tag||HG13822-NM|
|Human LMAN2L ORF mammalian expression plasmid, N-HA tag||HG13822-NY|
|Human LMAN2L natural ORF mammalian expression plasmid||HG13822-UT|
|Learn more about expression Vectors|
LMAN2L contains 1 L-type lectin-like domain and is expressed in numerous tissues. It is highly expressed in skeletal muscle and kidney, and its intermediate expression levels in heart, liver and placenta, low levels in brain, thymus, spleen, small intestine and lung. LMAN2L may be involved in the regulation of export from the endoplasmic reticulum of a subset of glycoproteins. It also may function as a regulator of ERGIC-53. LMAN2L gene may be modulated by acetylation; phosphorylation, as detailed at PhosphoSite. Alternative splicing produces 2 isoforms of the human protein. LMAN2L localize in various compartments.