|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human MARK3 ORF mammalian expression plasmid, C-GFPSpark tag||HG12181-ACG|
|Human MARK3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12181-ACR|
|Human MARK3 ORF mammalian expression plasmid, C-Flag tag||HG12181-CF|
|Human MARK3 ORF mammalian expression plasmid, C-His tag||HG12181-CH|
|Human MARK3 ORF mammalian expression plasmid, C-Myc tag||HG12181-CM|
|Human MARK3 ORF mammalian expression plasmid, C-HA tag||HG12181-CY|
|Human MARK3 Gene cDNA clone plasmid||HG12181-G|
|Human MARK3 ORF mammalian expression plasmid, N-Flag tag||HG12181-NF|
|Human MARK3 ORF mammalian expression plasmid, N-His tag||HG12181-NH|
|Human MARK3 ORF mammalian expression plasmid, N-Myc tag||HG12181-NM|
|Human MARK3 ORF mammalian expression plasmid, N-HA tag||HG12181-NY|
|Human MARK3 natural ORF mammalian expression plasmid||HG12181-UT|
|Learn more about expression Vectors|
MAP / microtubule affinity-regulating kinase 3, also known as C-TAK1, cTAK1, Cdc25C-associated protein kinase 1, ELKL motif kinase 2, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Serine/threonine-protein kinase p78, MARK3, CTAK1 and EMK2, is a ubiquitous expressed protein which belongs to the protein kinase superfamily, CAMK Ser / Thr protein kinase family and MARK subfamily. MARK3 contains one KA1 (kinase-associated) domain, one protein kinase domain and one UBA domain. The Par-1 / MARK protein kinases play a pivotal role in establishing cellular polarity. This family of kinases contains a unique domain architecture, in which a ubiquitin-associated (UBA) domain is located C-terminal to the kinase domain. MARKs / PAR-1 are common regulators of cell polarity that are conserved from nematode to human. All of these kinases have a highly conserved C-terminal domain, which is termed the kinase-associated domain 1 (KA1).