|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human AFP ORF mammalian expression plasmid, C-GFPSpark tag||HG12177-ACG|
|Human AFP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12177-ACR|
|Human AFP ORF mammalian expression plasmid, C-Flag tag||HG12177-CF|
|Human AFP ORF mammalian expression plasmid, C-His tag||HG12177-CH|
|Human AFP ORF mammalian expression plasmid, C-Myc tag||HG12177-CM|
|Human AFP ORF mammalian expression plasmid, C-HA tag||HG12177-CY|
|Human AFP Gene cDNA clone plasmid||HG12177-G|
|Human AFP ORF mammalian expression plasmid, N-Flag tag||HG12177-NF|
|Human AFP ORF mammalian expression plasmid, N-His tag||HG12177-NH|
|Human AFP ORF mammalian expression plasmid, N-Myc tag||HG12177-NM|
|Human AFP ORF mammalian expression plasmid, N-HA tag||HG12177-NY|
|Human AFP natural ORF mammalian expression plasmid||HG12177-UT|
|Learn more about expression Vectors|
Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).